glaucomaНациональный журнал ГлаукомаNational Journal glaucoma2078-41042311-6862Federal State Budgetary Institution of Science “Krasnov Research Institute of Eye Diseases”10.53432/2078-4104-2021-20-4-3-8glaucoma-352Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESМитохондриально-направленный антиоксидант SkQ1 предотвращает глаукомные повреждения у кроликовMitochondria-targeted antioxidant SkQ1 reverses glaucomatous lesions in rabbitsИомдинаЕ. Н.IomdinaЕ. N.

Москва, 105062

Moscow

Хорошилова-МасловаИ. П.Khoroshilova-MaslovaI. P.

Москва, 105062

Moscow

РобустоваО. В.RobustovaO. V.

Москва, 105062

Moscow

АверинаО. А.AverinaO. A.

Москва, 119992

Moscow 119992

КовалеваН. А.KovalevaN. A.

Moscow, 119992

Moscow 119992

АлиевГ.AlievG.

San Antonio, Texas, 78229; Johns Creek

San Antonio, Texas, 78229; Johns Creek

РеддиП. В.ReddyV. P.

Rolla, MO 65409

Rolla, MO 65409

Замятнин мл.А. А.Zamyatnin Jr.A. A.

Moscow, 119992; Москва, 19991

Moscow 119992; Moscow 119991

СкулачевМ. В.SkulachevM. V.

Москва, 119991

Moscow 11999

skulach@belozersky.msu.ruСенинИ. И.SeninI. I.

Москва, 119992

Moscow 119992

СкулачёвВ. П.SkulachevV. P.

Москва, 119992

Moscow 119992

Московский НИИ глазных болезней им. ГельмгольцаРоссияMoscow Helmholtz Research Institute of Eye DiseasesRussian FederationМосковский государственный университет имени М.В. Ломоносова, Факультет Биоинженерии и БиоинформатикиРоссияLomonosov Moscow State University, Faculty of Bioengineering and BioinformaticsRussian FederationМосковский государственный университет имени М.В. Ломоносова, Институт Физико-химической биологии им. БелозерскогоРоссияLomonosov Moscow State University, Belozersky Institute of Physico-Chemical BiologyRussian FederationGALLY International Biomedical Research Consulting LLC; School of Health Science and Healthcare Administration, The University of AtlantaСоединённые Штаты АмерикиGALLY International Biomedical Research Consulting LLC; School of Health Science and Healthcare Administration, The University of AtlantaUnited StatesDepartment of Chemistry, Missouri University of Science and TechnologyСоединённые Штаты АмерикиDepartment of Chemistry, Missouri University of Science and TechnologyUnited StatesМосковский государственный университет имени М.В. Ломоносова, Институт Физико-химической биологии им. Белозерского; Первый Московский государственный медицинский университет им. Сеченова, Институт молекулярной медициныРоссияLomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology; Sechenov First Moscow State Medical University, Institute of Molecular MedicineRussian FederationМосковский государственный университет имени М.В. Ломоносова, Биологический факультетРоссияLomonosov Moscow State University, Biological FacultyRussian FederationМосковский государственный университет имени М.В. Ломоносова, Институт Физико-химической биологии им. Белозерского; Московский государственный университет имени М.В. Ломоносова, Институт МитоинженерииРоссияLomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology; Lomonosov Moscow State University, Institute of MitoengineeringRussian FederationМосковский государственный университет имени М.В. Ломоносова, Факультет Биоинженерии и Биоинформатики; Московский государственный университет имени М.В. Ломоносова, Институт Физико-химической биологии им. БелозерскогоРоссияLomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics; Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical BiologyRussian Federation20212012202120438Copyright © Иомдина Е.Н., Хорошилова-Маслова И.П., Робустова О.В., Аверина О.А., Ковалева Н.А., Алиев Г., Редди П., Замятнин мл. А.А., Скулачев М.В., Сенин И.И., Скулачёв В.П., 20212021Иомдина Е.Н., Хорошилова-Маслова И.П., Робустова О.В., Аверина О.А., Ковалева Н.А., Алиев Г., Редди П., Замятнин мл. А.А., Скулачев М.В., Сенин И.И., Скулачёв В.П.Iomdina Е.N., Khoroshilova-Maslova I.P., Robustova O.V., Averina O.A., Kovaleva N.A., Aliev G., Reddy V., Zamyatnin Jr. A.A., Skulachev M.V., Senin I.I., Skulachev V.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.glaucomajournal.ru/jour/article/view/352

Оригинальная статья опубликована: Frontiers in Bioscience, Landmark, 20, 892-901, January 1, 2015.

Глаукома — основная причина необратимой слепоты в мире. Это заболевание характеризуется апоптозом ганглиозных клеток сетчатки (ГКС) и изменениями поля зрения, что, по-видимому, связано с повышенным внутриглазным давлением (ВГД). Есть основания полагать, что важную роль в патогенезе глаукомы играет митохондриальная дисфункция. Повышенный митохондриальный окислительный стресс в ГКС может лежать в основе или способствовать восприимчивости ГКС к апоптозу. В нашей работе мы (а) разработали модель хронического умеренно повышенного ВГД на кроликах для изучения глаукомы и (б) продемонстрировали эффективность митохондриально-направленного антиоксиданта SkQ1 в качестве инструмента для воздействия на некоторые проявления экспериментальной глаукомы, вызванной серией инъекций гидроксипропилметилцеллюлозы (ГПМЦ) в переднюю камеру глаза кролика. Показано, что инстилляции 0,2,5–5 мкМ раствора SkQ1 в течение 6-ти месяцев нормализуют ВГД и гидродинамику глаза, а также предотвращают увеличение толщины хрусталика, сопровождающее развитие экспериментальной глаукомы.

Original article is published in: Frontiers in Bioscience, Landmark, 20, 892–901, January 1, 2015

Glaucoma is the main cause of irreversible blindness worldwide. This disease is characterized by apoptosis of retinal ganglion cells (RGC) and visual field loss that seems to be related to elevated intraocular pressure (IOP). Several lines of evidences have implicated the crucial role of mitochondrial dysfunction in the pathogenesis of glaucoma. Increased mitochondrial oxidative stress in RGC may underlie or contribute to susceptibility of RGC to apoptosis. In our work we (i) designed a rabbit model of chronic, moderately elevated IOP for studying glaucoma and (ii) demonstrated efficacy of mitochondria-targeted antioxidant SkQ1 as a tool to reverse several traits of experimental glaucoma induced by a series of injections of hydroxypropylmethylcellulose (HPMC) to the anterior chamber of the rabbit eye. It is shown that 6 months instillations of drops of 0.2.5–5 µM solution of SkQ1 normalize IOP and eye hydrodynamics and abolish an increase in lens thickness that accompanies glaucoma.

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The authors declare that there are no conflicts of interest present.