Investigation of endothelin-1 levels in tear fluid in primary open-angle glaucoma
https://doi.org/10.53432/2078-4104-2025-24-1-8-13
Abstract
PURPOSE. To study endothelin-1 (ET-1) levels in tear fluid of patients with primary open-angle glaucoma (POAG) in relation to age, disease stage, and intraocular pressure (IOP).
METHODS. ET-1 levels in tear fluid were measured using enzyme-linked immunosorbent assay (ELISA) in 10 healthy volunteers and 61 patients with POAG (stages I–IV). The analysis was performed with Synergy MX photometer using ELISA Kit for Endothelin-1.
RESULTS. The normal ET-1 level in tear fluid was 8.57± 5.84 pg/ml. In POAG, ET-1 levels were 10.42±3.59 pg/ml at stage I, 28.70±6.11 pg/ml at stage II, 14.28±4.71 pg/ml at stage III, and 10.8±6.50 pg/ml at stage IV. After intensification of hypotensive treatment, IOP decreased from 18.55± 5.95 mm Hg to 13.27 mm Hg, correlating with a reduction in ET-1 levels from 22.83±4.82 to 12.31±2.49 pg/ml.
CONCLUSION. ET-1 is a significant systemic vasoconstrictor factor playing a key role in the pathogenesis of glaucomatous optic neuropathy. Moderate-stage glaucoma is characterized by a marked increase in ET-1 levels, which may serve as a prognostic marker. Intensified hypotensive therapy, associated with IOP reduction, correlates with a decrease in ET-1 levels.
About the Authors
S. Yu. Petrov
Helmholtz National Medical Research Center of Eye Diseases
Russian Federation
Russian Federation
Dr. Sci. (Med.), Head of the Glaucoma Department
14/19 Sadovaya-Chernogryazskaya St., Moscow, 105062
T. A. Pavlenko
Helmholtz National Medical Research Center of Eye Diseases
Russian Federation
Russian Federation
Cand. Sci. (Med.), Head of the Department of Pathophysiology and Biochemistry
14/19 Sadovaya-Chernogryazskaya St., Moscow, 105062
O. A. Lisovskaya
Helmholtz National Medical Research Center of Eye Diseases
Russian Federation
Russian Federation
researcher at the Department of Pathophysiology and Biochemistry
14/19 Sadovaya-Chernogryazskaya St., Moscow, 105062
O. I. Markelova
Helmholtz National Medical Research Center of Eye Diseases
Russian Federation
Russian Federation
post-graduate student
14/19 Sadovaya-Chernogryazskaya St., Moscow, 105062
A. D. Epkhieva
Helmholtz National Medical Research Center of Eye Diseases
Russian Federation
Russian Federation
post-graduate student
14/19 Sadovaya-Chernogryazskaya St., Moscow, 105062
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Review
For citations:
Petrov S.Yu., Pavlenko T.A., Lisovskaya O.A., Markelova O.I., Epkhieva A.D. Investigation of endothelin-1 levels in tear fluid in primary open-angle glaucoma. National Journal glaucoma. 2025;24(1):8-13. (In Russ.) https://doi.org/10.53432/2078-4104-2025-24-1-8-13
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