Morphological changes in the sclera after implantation of a new intrascleral implant in an ex vivo experiment
https://doi.org/10.53432/2078-4104-2026-25-2-3-9
Abstract
PURPOSE. To evaluate the morphological features of implantation of a new intrascleral implant (ISI) made of polymethyl methacrylate in an ex vivo experiment.
METHODS. The ex vivo experiment was conducted on scleral fragments obtained from the equatorial region of the eyes of cadaveric donors aged 40–60 years. The study group included eight scleral fragments in which the ISI was implanted during simulated glaucoma surgery (GS), while the control group consisted of eight scleral fragments subjected to GS without implantation. Organotypic cultivation was performed in a standard medium under normothermic conditions for 6 months. At 14, 30, 90, and 180 days, two fragments from each group were removed from the culture for scanning electron microscopy and immunohistochemical analysis.
RESULTS. Morphological findings demonstrated that the presence of the investigated ISI in cadaveric sclera did not induce an active proliferative cellular response throughout the entire observation period (up to 6 months).
CONCLUSION. The developed polymethyl methacrylate ISI shows promise for further clinical trials in glaucoma surgery.
About the Authors
K. I. Slastina
S.N. Fedorov National Medical Research Center "MNTK "Eye Microsurgery"
Russian Federation
Russian Federation
ophthalmologist, postgraduate student
59A Beskudnikovsky Blvd., Moscow, 127486
G. V. Sorokoletov
S.N. Fedorov National Medical Research Center "MNTK "Eye Microsurgery"
Russian Federation
Russian Federation
Dr. Sci. (Med.), Head of the Department of Glaucoma Surgery
59A Beskudnikovsky Blvd., Moscow, 127486
S. A. Borzenok
S.N. Fedorov National Medical Research Center "MNTK "Eye Microsurgery"
Russian Federation
Russian Federation
Dr. Sci. (Med.), Head of the Center for Fundamental and Applied Biomedical Problems
59A Beskudnikovsky Blvd., Moscow, 127486
D. S. Ostrovskiy
S.N. Fedorov National Medical Research Center "MNTK "Eye Microsurgery"
Russian Federation
Russian Federation
Cand. Sci. (Biol.), Head of the Laboratory of Transplantology and Cell Biology of the Center for Fundamental and Applied Biomedical Problems
59A Beskudnikovsky Blvd., Moscow, 127486
M. Kh. Khubetsova
S.N. Fedorov National Medical Research Center "MNTK "Eye Microsurgery"
Russian Federation
Russian Federation
Cand. Sci. (Med.), Head of the Eye Tissue Bank
59A Beskudnikovsky Blvd., Moscow, 127486
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Review
For citations:
Slastina K.I., Sorokoletov G.V., Borzenok S.A., Ostrovskiy D.S., Khubetsova M.Kh. Morphological changes in the sclera after implantation of a new intrascleral implant in an ex vivo experiment. National Journal glaucoma. 2026;25(2):3-9. (In Russ.) https://doi.org/10.53432/2078-4104-2026-25-2-3-9
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